Research Reveals How Chornobyl Wolves May Be Adapting to Chronic Radiation Exposure
By Cara Love, Molly Seltzer, and Tyjaha Steele

The research team works together to collect samples from a captured wolf in the Chornobyl Exclusion Zone. (Photo courtesy of James Beasley)
On April 28th, two days after the 40th anniversary of the Chernobyl nuclear explosion, a new paper was published in the journal, Molecular Ecology, that provides the most detailed look to date at the impact of widespread chronic radiation exposure on gene regulation and natural selection in the grey wolves living in the Chernobyl Exclusion Zone (CEZ).
The new research, conducted by a group of international scientists led by Princeton postdoctoral researcher Cara Love, suggests that wolves are rapidly evolving through natural selection to be able to survive in an environment where they are exposed to radiation levels roughly 250 times greater than those experienced by neighboring populations in Belarus, and well beyond the safety limit for the general human populous.

A radio collared wolf is seen moving through the Chornobyl Exclusion Zone. (Photo courtesy of James Beasley)
In 1986, a nuclear reactor at the Chernobyl power plant exploded, releasing large amounts of ionizing radiation into the surrounding environment in what became the world’s worst nuclear accident. The CEZ, a roughly 4,000 square kilometer area with elevated levels of radiation contamination and long abandoned by people, has been recolonized by numerous wildlife populations, including wolves, lynx, and Przewalskis horses, creating a unique opportunity for researchers to study how animals respond to long-term environmental exposure.
Using custom GPS collars equipped with a radiation dosimeter, a tool developed by professor Dr. Jim Beasley at the University of Georgia Savannah River Ecology Laboratory (SREL) and Dr. Thomas Hinton at Fukushima University to continuously measure individual exposure in real time, the researchers produced some of the most precise individual-level dose estimates ever recorded for individuals living within the CEZ. This work draws on more than a decade of field and laboratory collaboration with Dr. Stacey Lance, senior research scientist at SREL, and Dr. Beasley, and builds upon baseline ecological and exposure studies. The aim was to examine dose specific responses to chronic radiation exposure and explicitly examine evidence of radiation stress and natural selection acting on the immune system, a critical system for radiation stress response.
“While Cara (Dr. Love) was doing her PhD at UGA, we began to see signatures of radiation stress at a non-lethal level in the wolves and knew she was on to something exciting, and worthy of her further persuing this research,” states Dr. Lance.
Love et al. compared blood cell profiles of the CEZ wolves to those of reference populations in nearby Belarus and Yellowstone National Park. They found significant shifts in immune cell proportions, changes strikingly similar to those seen in cancer patients undergoing radiation treatment. The team also describe significant shifts in gene expression in CEZ wolves, particularly in pathways related to DNA damage response and immune signaling.
Given these signs of radiation stress, Love and colleagues examined evidence of adaptation within the immune system. They identified 15 genes associated with DNA damage repair and anti-tumor immunity that diverged significantly from grey wolf populations found outside the CEZ, suggesting these genes are targets of natural selection in response to radiation exposure within the CEZ.
“The genetic variants we’ve identified suggest that the CEZ wolves likely adapted to be able to better manage DNA damage stress caused by radiation exposure, which can be an underlying cause for cancer development,” says Cara Love, lead author on the paper. “This is the first time we can point to specific immune targets under active natural selection in response to a radiation-contaminated environment.”
The two genes that diverged most significantly from the reference populations, play important roles in DNA repair and have established roles in cancer immunology, making them noteworthy traits to look at in a population exposed to elevated radiation exposure across multiple generations.
The top candidate gene, APBB1IP, is a known regulator of innate and adaptive immune cell migration and is a prognostic biomarker in multiple cancers. Its expression in cancer patients predicts immune cell infiltration of tumors. Similarly, the second gene, EMC6, is a tumor suppressor linked to autophagy, apoptosis, and immune cell infiltration in gastric and lung cancer.
Researchers are seen processing a blood sample collected from a captured wolf. (Photo courtesy of James Beasley)
“Most of what we know about protective radiation and cancer genes comes from studying disease in people who are already sick. These wolves give us an opportunity to study resilience in a population that has been continuously challenged with an oncogenic stress for generations,” notes Cara Love.
“What we’re seeing in the wolves mirrors, to a large degree, what is seen in cancer patients undergoing radiation therapy, signs of persistent physiological stress. We still have much to learn, but we hope the changes we’re seeing in the Chernobyl wolves will lead to new options for treating cancer in humans” explains Shane Campbell-Staton, associate professor of ecology and evolutionary biology at Princeton and senior author on the new research.
The significance of these findings extends beyond wolves themselves, as the biological responses observed in the wolves may provide insight into broader questions surrounding radiation exposure, immune function, and cancer biology. According to the researchers, canids share many conserved immune and oncogenic pathways with humans, and dogs are already widely used as models for studying human cancers. This means the immune and genomic patterns observed in the wolves are likely to have meaningful parallels in human cancer biology and could ultimately inform how the scientific community thinks about radiation resistance and tumor immunity.
The full study, Signatures of Radiation-Induced Stress and Putative Selection on Immune Targets in Chornobyl Wolves, was published in Molecular Ecology. Authors include Cara N. Love, Stacey L. Lance, Thomas G. Hinton, Nicolas Rochette, James C. Beasley, Dmitry Shamovich, Michael E. Byrne, Brian Nadel, Sarah C. Webster, and Shane C. Campbell-Staton.















