SREL Reprint #3275

 

 

 

Twenty-four microsatellite markers for the gray mouse opossum (Tlacuatzin canescens): development from illumina paired-end sequences

Jesica Arcangeli1,2, Fernando A. Cervantes1, Stacey L. Lance3, Ma. Isabel Salazar4, and Jorge Ortega5

1Departamento de Zoologia, lnstituto de Biología, Universidad Nacional Autónoma de México,
Apartado Postal 70-153, 04510 Mexico, Distrito Federal, Mexico
2Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México,
Av. Universidad 3000, 04510 Mexico, Distrito Federal, Mexico
3Savannah River Ecology Laboratory, University of Georgia, Aiken, SC 29802, USA
4Laboratorio de Inmunología Celular e Inmunopatogénesis, Departamento de lnmunología,
Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de
Carpio y Plan de Ayala s/n, Col. Sto. Tomas, 11340 Mexico, Distrito Federal, Mexico
5Laboratorio de lctiología y Limnología, Posgrado en Ciencias Quimicobiológicas,
Departamento de Zoología, Escuela Nacional de Ciencias Biológicas,
lnstituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n,
Col. Sto. Tomas, 11340 Mexico, Distrito Federal, Mexico

Abstract: Tlacuatzin canescens is an opossum species endemic to Mexico from southern Sonora to Oaxaca, with populations in Tres Marías Islands and in central Peninsula of Yucatan. A technique based on Illumina paired-end sequencing of a library highly enriched for microsatellite repeats was used to develop loci. Twenty-four polymorphic (tri- and tetranucleotide) microsatellites were developed and tested as markers in the target species. All markers were genotyped on 18 different individuals from distinct locations. We observed medium to low genetic variation across most loci (mean number of alleles per locus = 8). Levels of expected heterozygosity across all markers was fairly low (mean HE= 0.477, mean HO = 0.480).

Keywords: Illumina, PAL_FINDER, Microsatellites, Tlacuatzin canescens

SREL Reprint #3275

Arcangeli, J., F. A. Cervantes, S. L. Lance, M. I. Salazar, and J. Ortega. 2013. Twenty-four microsatellite markers for the gray mouse opossum (Tlacuatzin canescens): development from illumina paired-end sequences. Conservation Genetic Resources 5(2): 367-370.

To request a reprint

 

 
SREL Home University of Georgia