SREL Reprint #3272

 

 

 

Development of microsatellite loci for the Honduran white-bat (Ectophylla alba) by using Illumina paired-end sequences

Alberto Morales-Leyva1, Rodrigo A. Medellín2, Stacey L. Lance3, Bernal Rodríguez-Herrera4,
Melina Del Real-Monroy1, and Jorge Ortega1

1Laboratorio de Bioconservación y Manejo, Posgrado en Ciencias Quimicobiológicas, Departamento
de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional,
Prolongación de Carpio y Plan de Ayala s/n, Col. Sto. Tomas, 11340 Mexico City, Mexico
2Laboratorio de Ecología y Conservación de Vertebrados, Departamento de Ecología de la Biodiversidad,
Instituto de Ecología, Universidad Nacional Autónoma de México, Circuito Exterior junto a Jardín Botánico,
04510 Mexico City, Mexico
3Savannah River Ecology Laboratory, University of Georgia, Aiken, SC 29802, USA
4Sección de Zoología, Escuela de Biología, Universidad de Costa Rica, San Pedro Montes de Oca,
San José, Costa Rica

Abstract: Ectophylla alba is a bat restricted to Costa Rica, Honduras, Nicaragua and Panamá. A technique based on Illumina paired-end sequencing of a library highly enriched for microsatellite repeats was used to develop loci. Thirteen polymorphic (tri, tetra- and pentanucleotide) microsatellites were developed and tested. All markers were genotyped on 42 different individuals from 5 distinct locations. We observed low to medium–high genetic variation across most loci. Levels of expected heterozygosity across all markers was medium to low (mean HE = 0.659, mean HO = 0.672).

Keywords: Ectophylla alba, Illumina, Microsatellite, PAL_FINDER

SREL Reprint #3272

Morales-Leyva, A., R. A. Medellín, S. L. Lance, B. Rodríguez-Herrera, M. Del Real-Monroy, and J. Ortega. 2014. Development of microsatellite loci for the Honduran white-bat (Ectophylla alba) by using Illumina paired-end sequences. Conservation Genetic Resources 6(1): 219-220.

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