Jenica M. Allen¹, Samuel G. Obae², Mark H. Brand², John A. Silander¹,
Kenneth L. Jones³, Schyler O. Nunziata⁴, and Stacey L. Lance⁴

¹Department of Ecology and Evolutionary Biology, University of Connecticut,
Storrs, Connecticut 06269 USA
²Department of Plant Science and Landscape Architecture, University of Connecticut,
Storrs, Connecticut 06269 USA
³Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine,
Aurora, Colorado 80045 USA
⁴Savannah River Ecology Laboratory, University of Georgia, Aiken, South Carolina 29802 USA

Premise of the study: Microsatellite markers were isolated and characterized in Berberis thunbergii, an invasive and ornamental shrub in the eastern United States, to assess genetic diversity among populations and potentially identify horticultural cultivars.
Methods and Results: A total of 12 loci were identified for the species. Eight of the loci were polymorphic and were screened in 24 individuals from two native (Tochigi and Ibaraki prefectures, Japan) and one invasive (Connecticut, USA) population and 21 horticultural cultivars. The number of alleles per locus ranged from three to seven, and observed heterozygosity ranged from 0.048 to 0.636.
Conclusions: These new markers will provide tools for examining genetic relatedness of B. thunbergii plants in the native and invasive range, including phylogeographic studies and assessment of rapid evolution in the invasive range. These markers may also provide tools for examining hybridization with other related species in the invasive range.

Keywords: Berberidaceae; Berberis thunbergii; invasive; microsatellite; PCR primers; simple sequence repeat markers; short tandem repeat

SREL Reprint #3221