Development and characterization of 12 microsatellite loci for the Dwarf Salamander, Eurycea quadridigitata

Anna M. Mckee^1,2, Stacey L. Lance³, Kenneth L. Jones⁴, Cris Hagen³, and Travis C. Glenn^2,4

¹Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602, USA
²Department of Environmental Health Science, University of Georgia, Athens, GA 30602, USA
³Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29802, USA
⁴Georgia Genomics Facility, University of Georgia, Athens, GA 30602, USA

Abstract: We isolated and characterized 12 microsatellite loci for the Dwarf Salamander, Eurycea quadridigitata. Loci were screened in 30 individuals of E. quadridigitata. The number of alleles per locus ranged from 2 to 9, observed heterozygosity ranged from 0.2 to 0.9, and the probability of identity values ranged from 0.034 to 0.689. These new loci can be used to study population genetic structure and potentially help determine cryptic species divergence.