Ten novel microsatellite markers for the western mosquitofish Gambusia affinis

K. M. Purcell¹, S. L. Lance², K. L. Jones³, and C. A. Stockwell¹

¹Department of Biological Sciences, North Dakota State University, NDSU Dept. 2715,
218 Stevens Hall, P.O. Box 6050, Fargo, ND 58108-6050, USA
²Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29802, USA
³Georgia Genomics Facility, University of Georgia, Athens, GA 30602, USA

Abstract: We isolated and characterized 10 microsatellite loci from the western mosquitofish, Gambusia affinis. Loci were screened in 30 individuals from a single location from their native range in coastal Texas. The number of alleles per locus ranged from 4 to 25, observed heterozygosity ranged from 0.40 to 0.93, and the probability of identity values from 6.0 × 10^-3 to 2.7 × 10^-1, with an overall PI for all loci of 1.3 × 10^-13. These new loci provide tools for examining the genetic diversity, structure, and pedigree G. affinis populations.