SREL Reprint #3157
Development and characterization of twelve polymorphic microsatellite loci in the Bog Copper, Lycaena epixanthe
Sarah P. Flanagan1, W. Herbert Wilson1, Kenneth L. Jones2, and Stacey L. Lance3
1Department
of Biology,
Colby College,
Waterville,
ME 04901,
USA
2Georgia
Genomics
Facility,
University
of Georgia,
Athens,
GA 30602,
USA
3Savannah
River Ecology
Laboratory,
University
of Georgia,
Drawer E,
Aiken, SC
29802, USA
Abstract: We isolated and characterized 12 microsatellite loci from the Bog Copper, Lycaena epixanthe. Loci were screened in 24 individuals from one Maine location. Alleles per locus ranged from 3 to 12, observed heterozygosity ranged from 0.042 to 0.857, and the probability of identity values ranged from 0.032 to 0.780. These loci provide tools for examining the population genetics of Bog Coppers across a fragmented landscape.
Keywords: Bog Copper, Butterfly, Lycaena, Microsatellite, PCR primers, SSR, STR
SREL Reprint #3157
Flanagan, S. P., W. H. Wilson, K. L. Jones, and S. L. Lance. 2010. Development and characterization of twelve polymorphic microsatellite loci in the Bog Copper, Lycaena epixanthe. Conservation Genetics Resources 2010(2): 159-161.