SREL Reprint #3153
Fifteen
microsatellite
loci
for
the
decollate
snail,
Rumina
decollata
Stacey L. Lance1, Kenneth L. Jones2, Cris Hagen1, Kurt Jordaens3,4, Thierry Backeljau4,5, and Vanya Prévot5,6
1Savannah
River
Ecology
Laboratory,
University
of
Georgia,
Aiken,
SC
29802,
USA
2Georgia
Genomics
Facility,
University
of
Georgia,
Athens,
GA
30602,
USA
3Royal
Museum
for
Central
Africa,
Leuvensesteenweg
13,
3080
Tervuren,
Belgium
4Evolutionary
Ecology
Group,
University
of
Antwerp,
Groenenborgerlaan
171,
2020
Antwerp,
Belgium
5Royal
Belgian
Institute
of
Natural
Sciences,
Vautierstraat
29,
1000
Brussels,
Belgium
6Evolutionary
Biology
and
Ecology,
Université
Libre
de
Bruxelles
(CP
160/12),
Av.
F.D.
Roosevelt,
50,
1050
Brussels,
Belgium
Abstract: We characterized 15 microsatellite loci from the decollate snail, Rumina decollata. Loci were screened in 21 individuals and several individuals of the congener Rumina saharica. There was ample allelic diversity (6–12 alleles per locus) but observed heterozygosity values were extremely low (0–0.421). This was expected given the high self-fertilization rate in this species. Ten of the 15 loci were successfully amplified in R. saharica. These loci provide tools for examining the population genetics and taxomomic boundaries in R. decollata and its allies.
Keywords: Rumina, Land snail, Microsatellite, PCR primers, SSR, STR
SREL Reprint #3153
Lance, S. L., K. L. Jones, C. Hagen, K. Jordaens, T. Backeljau, and V. Prévot. 2010. Fifteen microsatellite loci for the decollate snail, Rumina decollata. Conservation Genetics Resources 2010(2): 287-289.