SREL Reprint #3127
Development and characterization of twelve polymorphic microsatellite loci in the threatened Red Hills salamander, Phaeognathus hubrichti
Stacey L. Lance1,2, Cris Hagen2, Travis C. Glenn1, Joseph J. Apodaca3, and Leslie J. Rissler3
1Department
of Environmental Health Science, University of Georgia, Athens, GA
30602, USA
2Savannah River Ecology Laboratory, University of Georgia,
Drawer E, Aiken, SC 29802, USA
3Department of Biological Sciences, University of Alabama,
Tuscaloosa, AL 35487, USA
Abstract: We isolated and characterized 12 microsatellite loci from the endangered Red Hills salamander, Phaeognathus hubrichti. Loci were screened in 24 individuals of P. hubrichti. The number of alleles per locus ranged from 2 to 16, observed heterozygosity ranged from 0.130 to 0.750, and the probability of identity values ranged from 0.012 to 0.778. These new loci provide tools for examining the population genetics of this federally threatened salamander.
Key words: Red Hills salamander, Phaeognathus, Microsatellite, PCR primers, SSR, STR
SREL Reprint #3127
Lance, S. L., C. Hagen, T. C. Glenn, J. J. Apodaca, and L. J. Rissler. 2009. Development and characterization of twelve polymorphic microsatellite loci in the threatened Red Hills salamander, Phaeognathus hubrichti. Conservation Genetics 10: 1919-1921.