SREL Reprint #3109
253 Novel polymorphic microsatellites for the saltwater crocodile (Crocodylus porosus)
Lee G. Miles1, Sally R. Isberg1,2, Chris Moran1, Cris Hagen3, and Travis C. Glenn3,4
1Faculty
of Veterinary Science, University of Sydney, Room 513, RMC Gunn Building,
Sydney, NSW 2006, Australia
2Porosus Pty Ltd, P.O. Box 86, Palmerston, NT 0831, Australia
3Savannah River Ecology Laboratory, University of Georgia,
P.O. Drawer E, Aiken, SC 29802, USA
4Department of Environmental Health Science, University of
Georgia, Athens, GA 30602, USA
Abstract: Genomic elucidation and mapping of novel organisms requires the generation of large genetic resources. In this study, 253 novel and polymorphic microsatellite loci were isolated and characterized for the saltwater crocodile (Crocodylus porosus) by constructing libraries enriched for microsatellite DNA. All markers were evaluated on animals obtained from Darwin Crocodile Farm in the Northern Territory, Australia, and are intended for future use in the construction of a genetic-linkage map for the saltwater crocodile. The 253 loci yielded an average of 4.12 alleles per locus, and those selected for mapping had an average polymorphic information content (PIC) of 0.425.
Keywords: Estuarine crocodile, Reptile, SSRs, Primers, Tetra-nucleotide repeats, Tri-nucleotide repeats, Di-nucleotide repeats, Enrichment
SREL Reprint #3109
Miles, L. G., S. R. Isberg, C. Moran, C. Hagen, and T. C. Glenn. 2009. 253 Novel polymorphic microsatellites for the saltwater crocodile (Crocodylus porosus). Conservation Genetics 10(2009): 963-980.