Isolation and characterization of microsatellite loci in the Guanacaste tree, Enterolobium cyclocarpum

MAUREEN B. PETERS¹, CRIS HAGEN¹, DORSET W. TRAPNELL², J. L. HAMRICK³,
OSCAR ROCHA⁴, PETER E. SMOUSE⁵, and TRAVIS C. GLENN¹

¹Savannah River Ecology Laboratory, University of Georgia, PO Drawer E, Aiken, SC 29802, USA
²Institute of Ecology, University of Georgia, Athens, GA 30602, USA
³Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
⁴Department of Biological Sciences, Kent State University, Kent, OH 44242, US
⁵Ecology, Evolution and Natural Resources, Rutgers University, New Brunswick, NJ 08901, USA

Abstract: We isolated nine microsatellite loci from the Guanacaste tree (Enterolobium cyclocarpum) and optimized them for future research on breeding populations of this species. Loci were screened across 53 individuals from one population and were shown to be variable with the number of alleles per locus ranging from five to 15. Polymorphic information content ranged from 0.420 to 0.900 and observed heterozygosity ranged from 0.547 to 0.906.