SREL Reprint #2841
Tetranucleotide, trinucleotide, and dinucleotide loci from the bobcat (Lynx rufus)
Brant
C. Faircloth1, Allison Reid1, Teresa Valentine1,
Soo Hyung Eo1, Theron M. Terhune1, Travis C. Glenn2,3,
William E. Palmer4, Campbell Joseph Nairn1, and
John P. Carroll1
1D. B. Warnell School of Forest Resources, University of Georgia,
Athens, GA, 30602, USA
2Savannah River Ecology Laboratory, University of Georgia,
P.O. Drawer E, Aiken, SC 29802, USA
3Department of Biological Sciences, University of South Carolina,
Columbia, SC 29208, USA
4Tall Timbers Research Station, 13093 Henry Beadel Drive, Tallahassee,
FL 32312, USA
Abstract: We describe primers and polymerase chain reaction (PCR) conditions to amplify four dinucleotide, one trinucleotide, and three tetranucleotide microsatellite DNA loci from the bobcat (Lynx rutus). The primers were tested on 22 individuals collected from a population located within southwestern Georgia (USA). The primer pairs developed in this study yielded an average of 7.4 alleles per locus (range four to 10), an average observed heterozygosity of 0.60 (range 0.40 to 0.76), and an average polymorphic information content of 0.70 (range 0.51 to 0.78).
Keywords: bobcat, dinucleotide repeats, Lynx rufus, microsatellites, primers, SSRs, tetranucleotide repeats, trinucleotide repeats
SREL Reprint #2841
Faircloth, B. C., A. Reid, T. Valentine, S. H. Eo, T. M. Terhune, T. C. Glenn, W. E. Palmer, C. J. Nairn, and J. P. Carroll. 2005. Tetranucleotide, trinucleotide, and dinucleotide loci from the bobcat (Lynx rufus). Molecular Ecology Notes 5:387-389.