SREL Reprint #2840

Isolation and characterization of microsatellite markers in the East African tree, Acacia brevispica (Fabaceae: Mimosoideae)

Adriana Otero-Arnaiz¹, Andrew Schnabel¹, Travis C. Glenn², Nancy A. Schable², Cris Hagen²,
and Leila Ndong¹

¹Department of Biological Sciences, Indiana University South Bend, PO Box 7111,
South Bend, IN 46634, USA
²Savannah River Ecology Laboratory, Drawer E, Aiken, SC 29802, USA

Abstract: We have developed primer pairs for 24 microsatellite loci isolated from Acacia brevispica, which is widespread in woodland savannas of East Africa. The loci were screened for levels of variation using 16-52 individuals from Mpala Research Centre, Kenya. Number of alleles per locus ranged from two to 17, and polymorphic information content ranged from 0.248 to 0.864. Several loci showed the presence null alleles, but many loci will be useful in ongoing studies of genetic structure, gene flow, breeding systems, and natural selection.

Keywords: Acacia, gene flow, genetic structure, molecular markers, PCR, SSR

SREL Reprint #2840

Otero-Arnaiz, A., A. Schnabel, T. C. Glenn, N. A. Schable, C. Hagen, and L. Ndong. 2005. Isolation and characterization of microsatellite markers in the East African tree, Acacia brevispica (Fabaceae: Mimosoideae). Molecular Ecology Notes 5:366-368.

To request a reprint