SREL Reprint #2838

 

 

 

Characterization of six microsatellite primers for the grey fox (Urocyon cinereoargenteus)

Julie L. Weston1,2, Nancy A. Schable1, and Travis C. Glenn1,2

1Savannah River Ecology Laboratory, P.O. Drawer E, Aiken, SC 29802, USA
2Peromyscus Genetic Stock Center, Department of Biological Sciences,
University of South Carolina, Columbia, SC 29208, USA

Abstract: We describe polymerase chain reaction (PCR) primers and conditions to amplify one dinucleotide and five tetranucleotide microsatellite DNA loci isolated from the grey fox (Urocyon cinereoargenteus). The PCR primers were tested on nine to 12 individuals collected from the Department of Energy's Savannah River site (Aiken, SC, USA). The grey fox microsatellite primers developed had three to 10 alleles per locus that yielded observed heterozygosities ranging from 0.222 to 0.889.

Keywords: dinucleotide repeats, grey fox, microsatellite, polymerase chain reaction, primer, simple sequence repeat, tetranucleotide repeats, Urocyon cinereoargenteus

SREL Reprint #2838

Weston, J. L., N. A. Schable, and T. C. Glenn. 2004. Characterization of six microsatellite primers for the grey fox (Urocyon cinereoargenteus). Molecular Ecology Notes 4:503-505.

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