SREL Reprint #2676




Carbon and hydrogen isotope fractionations associated with dissimilatory iron-reducing bacteria

Christopher S. Romanek1, Chuanlun L. Zhang2, Yiliang Li2, Juske Horita3, H. Valid4, David R. Cole3,
Tommy J. Phelps5

1Department of Geology and the Savannah River Ecology Laboratory, University of Georgia,
Drawer E, Aiken, SC 29802, USA
2Department of Geological Sciences, University of Missouri, 101 Geological Sciences Building,
Columbia, MO 65211, USA
3Chemical and Analytical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA
4Department of Earth and Planetary Sciences, McGill University, 3450 University Street,
Montreal, QC, Canada H3A 2C7
5Environmental Siences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA

Abstract: Shewanella putrefaciens strain CN-32 and Shewanella algae strain BrY were grown in laboratory cultures at 30°C to characterize carbon and hydrogen isotope fractionation patterns related to the growth of iron-reducing bacteria. Ferric citrate or hydrous ferric oxide (HFO) was provided as the electron acceptor and lactate or H2 (balanced with CO2) was used as the electron donor. Because these bacteria are not known to grow chemoautotrophically, yeast extract was provided as a carbon source when cultures were grown on H2/CO2.
Siderite formed only when HFO was used as the electron acceptor, possibly because of chelation of ferrous iron with dissolved citrate when ferric citrate was used as the electron acceptor. Carbon isotope enrichment factors for the siderite-CO2 system (εsid-CO2) ranged from 13.3‰ to 14.5‰ when lactate was used as the carbon and energy source, which were consistent with theoretical calculations of equilibrium isotope fractionation (αsid-CO2) for the siderite-CO2 system [Geochim. Int. 18 (1981) 85]. In experiments using H2/CO2 as the energy source and yeast extract as the carbon source, carbon isotope enrichment factors were relatively low (0.5‰ to 7.4‰). The potential exists that a kinetic effect related to siderite precipitation rate influenced isotope partitioning or a dynamic balance was established between carbon sinks (i.e. biomass and solid carbonate) of diverging carbon isotope composition. A more quantitative estimate of εsid-CO2 for biological systems that contain ambient dissolved inorganic carbon (DIC) requires a deeper understanding of carbon flow dynamics in these compartmentalized closed systems.
Finally, in experiments using H2/CO2 as an energy source, the hydrogen isotope composition of head gas H2 and water were analyzed for D/H ratio. The results indicate that bacterial metabolism potentially facilitates isotope exchange between water and H2.

Keywords: Iron-reducing bacteria; Biogenic siderite; Carbon isotopes; Hydrogen isotopes

SREL Reprint #2676

Romanek, C. S., C. L. Zhang, Y. Li, J. Horita, H. Vali, D. R. Cole, and T. J. Phelps. 2003. Carbon and hydrogen isotope fractionations associated with dissimilatory iron-reducing bacteria. Chemical Geology (Isotope Geoscience) 195:5-16.

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