SREL Reprint #1981

 

 

 

 

Electrophoretic Comparison of Road-killed Deer and Live-captured Deer Sampled by Muscle Biopsy

Mary J. Ratnaswamy, Daniel B. Wamell School of Forest Resources, University of Georgia, Athens, GA 30602
Carolyn L. Rogers, Daniel B. Wamell School of Forest Resources, University of Georgia, Athens, GA 30602
Robert J. Warren, Daniel B. Wamell School of Forest Resources, University of Georgia, Athens, GA 30602
Michael H. Smith, Savannah River Ecology Laboratory, Drawer E, Aiken, SC 29802 and Departments of Genetics and Zoology, Institute of Ecology, Daniel B. Wamell School of Forest Resources, University of Georgia, Athens, GA 30602
Karl A. K. Stromayer, Daniel B. Wamell School of Forest Resources, University of Georgia, Athens, GA 30602

Abstract: A safe and effective muscle biopsy procedure that can be used to sample genetic variation in live white-tailed deer (Odocoileus virginianus) is described. The validity of this procedure for estimating genetic variability was confirmed by sampling blood and muscle from 78 road-killed and 57 captured deer at Chickamauga Battlefield National Military Park, Georgia, between June 1991 and June 1992. Six polymorphic loci were detected in muscle tissue and 2 polymorphic loci were found in blood using starch-gel electrophoresis. We compared levels of genetic variation in 3 sampling groups: road-killed deer, "actively" captured deer, and "passively" captured deer. Deer were considered "active" captures if the capture method did not involve baiting at the capture site (i.e., drive-nets, poaching, scientific collections, remote darting). Deer were classified as "passive" captures if bait was used to attract deer to the capture location (e.g., drop-nets, corral-drive nets, remote darting over bait). No significant differences were found between road-killed and captured deer for mean heterozygosity, mean number of alieles, or average aliele frequencies.

SREL Reprint #1981

Ratnaswamy, M.J., C.L. Rogers, R.J. Warren, M.H. Smith, and K.A.K. Stromayer. 1993. Electrophoretic comparison of road killed deer and live captured deer sampled by muscle biopsy. Proceedings of the Annual Conference of the Southeastern Association of Fish and Wildlife Agencies 47:211-221.

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