SREL Reprint #1817






R.L. Brigmonl, H.W. Martin2, and S.G. Zam3
1Departments of Physiological Science and 3Microbiology and Cell Science, University of Florida, Gainesville, FL 32610
2Savannah River Ecology Laboratory, University of Georgia, Aiken, SC 29802

Immunocytochemical techniques utilizing monoclonal antibodies (MAb), electron microscopy (EM), and microbiological procedures have been developed for the study of Thiothrix spp. in aquatic systems. An enzyme-linked immunosorbent assay (ELISA) was developed incorporating a Thiothrix-specific MAb. The ELISA utilizing the MAb is a useful tool to classify and help understand the complex nature of Thiothrix spp. The ELISA is fast, accurate, and results were not different from microscopy, staining, and biochemical procedures. The MAb was incorporated with EM cytochemical techniques using colloidal gold for further studies of Thiotlirix ultrastructure and identification in natural and isolated cultures.

SREL Reprint #1817

Brigmon, R.L., H.W. Martin, and S.G. Zam. 1994. Microbial ecology of Thiothrix spp. in the Floridan aquifer. p. 4-5. In Breakthroughs in Karst Geomicrobiology and Redox Geochemistry, edited by I.D. Sasowsky and M.V. Palmer. Karst Waters Institute. Colorado Springs, CO.

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